Optimization of the porcine adult skin-derived precursor cell isolation protocol
نویسندگان
چکیده
منابع مشابه
Neuro-Muscular Differentiation of Adult Porcine Skin Derived Stem Cell-Like Cells
BACKGROUND Due to the genetic relationship to humans, porcine stem cells are a very important model system to investigate cell differentiation, associated cell signaling pathways, and cell fate. Porcine skin derived stem cells have been isolated from mid-gestation porcine fetus recently. To our knowledge, stem cells from the skin of the adult porcine organism have not been isolated until now. H...
متن کاملRapid isolation and expansion of skin-derived precursor cells from human primary fibroblast cultures
Skin-derived precursor (SKP) cells have self-renewal and multipotent abilities and are found in the dermis. SKP cells have been isolated previously from pre-established dermal fibroblast cultures. In these procedures, long-term culture and low yield remain the crucial aspects requiring improvement. In this study, we exposed pre-established dermal fibroblasts to 30-min acid stress prior to isola...
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In the present study, the effect of two types of allogeneic-derived feeder cells [porcine ear and tail skin fibroblasts (PESF, PTSF)] and three types of xenogeneic-derived feeder cells [human foreskin fibroblasts (HFK), mouse embryonic fibroblasts (MEF) and immortalized mouse embryonic fibroblasts (STO)] on the isolation and cultivation of putative porcine embryonic stem cells (pESCs) was evalu...
متن کاملSkin-derived precursor cells promote wound healing in diabetic mice.
BACKGROUND Impaired wound healing as one of the complications arising from diabetes mellitus is a serious clinical issue. Recently, various cell therapies have been reported for promotion of wound healing. Skin-derived precursor cells (SKPs) are multipotent adult stem cells with the tendency to differentiate into neurons. We investigated the potency of promoting diabetic wound healing by the ap...
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ژورنال
عنوان ژورنال: Stem Cell Discovery
سال: 2013
ISSN: 2161-6760,2161-6787
DOI: 10.4236/scd.2013.31011